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KMID : 0861020090240030097
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Korea Journal of Herbology
2009 Volume.24 No. 3 p.97 ~ p.102
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Anti-inflammatory Effects of Herbal Formula KCNS-001 for Mitigating Atopic Dermatitis
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Lee Jung-Bok
Choi Jae-Hwan
Bang Ok-Sun
Yu Young-Beob
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Abstract
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Objectives : We determined the anti-inflammatory activity of KCNS-001 that is a herbal formula including 6 medicinal plants and that are used to mitigate atopic dermatitis in oriental medicine.
Methods : To evaluate anti-inflammatory effect of KCNS-001, we measured the production of reactive oxygen species (ROS), nitric oxide (NO) and cyclooxygenase-2 (COX-2) in LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF- were detected by enzyme immuno assay (EIA) and enzyme-linked immunosorbent assay (ELISA).
Results : ROS and NO production were reduced by KCNS-001 in a dose-dependent manner. KCNS-001 significantly inhibited activity of COX-2 and suppressed the release of tumor necrosis factor-alpha (TNF-).
Conclusions : These results indicate that the KCNS-001 may have an anti-inflammatory agent for the treatment of various inflammatory disease.
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KEYWORD
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KCNS-001(herbal formula), ROS, NO, COX-2, TNF-, LPS, Raw 264.7
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